Presence of Streptococcus mutans and interleukin-6 and -10 in amniotic fluid.

AIM: To evaluate the presence of Streptococcus mutans (SM), main pathogen of dental caries, interleukin-6 and -10 in samples of amniotic fluid (AF) and clinical and oral data of the healthy pregnancy, to investigate the SM exposition in intrauterine life and to elucidate the presence of antibodies to SM in newborns' saliva (SA) with undetectable levels of this microorganism described in the previous study. METHODS: The study involved the participation of 26 women with term deliveries and without gestational complications. General health data were collected through a questionnaire applied to the participants. The volunteers were examined orally, the caries experience (decayed, missing, and filled teeth (DMFT) scores) was calculated and unstimulated SA was collected. The acquisition of AF was performed during cesarean sections and stocked on ice. The analysis of the presence of SM-DNA was performed by real-time PCR assays with specific primers. Levels of IL-6 and IL-10 were performed by ELISA with specific kits for these interleukins in the AF samples. RESULTS: 69.2% of the AF samples had detectable SM, and 65% of the women had the bacteria in the AF and SA at the same time. There was a positive association between the presence of SM in AF and a report of not going to the dentist frequently (p<.05). There was no statistically significant difference between the frequency of AF with or without detection of MS and active caries (p>.05). However, the DMFT scores of women with positive AF for SM were higher than that of women without detectable MS (p<.05). IL-6 levels were higher than IL-10 in AF (p<.05). There were no significant differences in the levels of interleukins between samples with detectable SM or in AF and even with clinical data analysis (p>.05). CONCLUSIONS: The AF samples have SM in quantifiable levels, probably associated with the greatest caries experience, and seen by the highest DMFT index. Interleukin levels were not related to the presence of MS. The presence of SM in AF may explain the mucosal immune stimulation of newborn children by the previous detection of salivary antibodies against SM.

Development of Streptococcus mutans biofilm in the presence of human colostrum and 3'-sialyllactose.

AIM: To evaluate the initial adhesion and formation of Streptococcus mutans biofilm in vitro in the presence of saliva, human colostrum and 3'-sialyllactose. METHODS: Human colostrum and salivas were collected from 30 mothers and newborn postpartum. Eighteen hours culture of S. mutans was treated with colostrum or 3'-sialyllactose in three different moments: before, during, and after 24 h from the microbial inoculation. Salivas were also tested in conjunction with colostrum. The assays were realized in sterile 96-well flat-bottom microtiter plates for 24 h. The biofilms were fixed, washed, stained with crystal violet, and extracted. Absorbance was measured to evaluate biofilm growth mass. RESULTS: Colostrum applied after and during the inoculation decreased biofilm formation when compared with the control (p < .05). The presence of saliva increased the biofilm biomass (p < .05). The application of 3'-sialyllactose reduced biofilm formation independently of moments of application (p < .05). CONCLUSION: Saliva contributed to the proliferation of biofilm and colostrum did not prevent the initial adhesion, but interfered in the accumulation and development of microorganisms in biofilms. 3'-sialyllactose significantly decreased biofilm formation. This information expands the importance of colostrum as a potent oral antimicrobial biofluid.

Color Stability and Surface Roughness of Hybrid and Nanofilled Composites Brushed with Abrasive Dentifrices / Estabilidade de Cor e Rugosidade Superficial de Resinas Compostas Micro-Híbridas e Nanoparticulada Submetidas à Escovação com Dentifrícios Abrasivos

The aim of this study was to evaluate the color stability and the surface roughness of different composites brushed with toothpastes presenting different levels of abrasivity. Thirty discs of each material were obtained using michohybrid composites (Brilliant NG and Charisma Diamond) and a nanocomposite (Filtek Z350XT). The initial color (CIELab) and surface roughness (confocal laser scanning microscopy) of resin discs were evaluated. Afterwards, 10 specimens per group were brushed with the following dentifrices: Maximum Cavity Protection, Sensodyne Repair & Protect and Colgate Sensitive Pro-Relief. Brushing was performed with an electric toothbrush equipped with soft bristle head, with standard power and weight, for 30 minutes. Every 30 seconds, 1.0 ml of the slurry was injected between the bristles of the brush and the specimen. After abrasive challenge, the samples had their color and roughness reevaluated. Data were submitted to the Kruskal-Wallis test (color change) or the t-test (surface roughness). The level of significance was 5%. Results: Brushing did not significantly change the color of the composites tested in the study herein . On the other hand, the surface roughness of the composites was significantly affected by the abrasive challenge, regardless of the toothpaste used. The surface roughness change was similar for all the composites. The abrasive challenge with the toothpastes Maximum Cavity protection, Sensodyne Repair & Protect and Colgate Sensitive Pro-Relief was not able to significantly change the color of the composite resins. Nevertheless, the abrasive challenges significantly altered the surface roughness of all the evaluated composites. However, the changes in surface roughness were statistically similar in the microhybrid and nanofilled composites. (AU)

O objetivo deste estudo foi avaliar a estabilidade de cor e a rugosidade superficial de diferentes resinas compostas escovadas com dentifrícios de diferentes níveis de abrasividade. Trinta discos de cada material foram obtidos utilizando compósitos micro-híbridos (Brilliant NG e Charisma Diamond) e um nanocompósito (Filtek Z350XT). A cor inicial (CIELab) e a rugosidade superficial (microscopia confocal de varredura a laser) dos discos de resina foram avaliadas. Em seguida, 10 amostras por grupo foram escovadas com os dentifrícios Máxima Proteção Anticáries, Sensodyne Repair & Protect e Colgate Sensitive Pro-Alívio. A escovação foi realizada com uma escova elétrica com cabeça de cerdas macias, com potência e peso padronizados, durante 30 minutos. A cada 30 segundos, 1,0 ml da pasta era injetada entre as cerdas da escova e a amostra. Após o desafio abrasivo, as amostras tiveram sua cor e rugosidade reavaliadas. Os dados foram submetidos ao teste de Kruskal-Wallis (alteração de cor) ou ao teste t (rugosidade da superfície) (α=5%). A escovação não alterou significativamente a cor dos compósitos. Por outro lado, a rugosidade superficial dos compósitos foi significativamente afetada pelo desafio abrasivo, independentemente do dentifrício utilizado. A alteração da rugosidade superficial foi semelhante para todos os compósitos. O desafio abrasivo com a Máxima Proteção Anticáries, o Sensodyne Repair & Protect e o Colgate Sensitive Pro-Alívio não foi capaz de alterar significativamente a cor das resinas. Diferentemente, os desafios abrasivos alteraram significativamente a rugosidade superficial de todos os compósitos avaliados. No entanto, as mudanças na rugosidade foram estatisticamente semelhantes nos compósitos micro-híbridos e nanoparticulado. (AU)

Overexpression of HIF-1α and Morphological Alterations in the Tongue of Rats Exposed To Secondhand Smoke.

Smoking is a risk factor for serious health problems and is associated with several changes in the tissues of the oral cavity. The aim of this study was to evaluate the collagen percentage, mast cells density, intensity of immunolabeled cells by anti-HIF-1α in the musculature lingual of rats exposed to secondhand smoke. Twenty-seven female Wistar albino rats were divided into three groups: rats not exposed to tobacco smoke inhalation (Control group) (n=7); rats exposed to smoke inhalation for 30 days (TAB 30) (n=10); and rats exposed to smoke inhalation for 45 days (TAB 45) (n=10). Subsequently, the animals were submitted to euthanasia and removal of the tongue for histological and immunohistochemistry processing and analysis. In the groups TAB 30 and TAB 45 there were a lower percentage of collagen, a higher density of mast cells and a greater intensity of anti-HIF-1α immunolabeled cells compared to Control group. There was also a positive and significant correlation between the percentage of collagen and mast cell density. There was not significative difference between TAB 30 e TAB 45 in any of the parameters evaluated. Therefore, the exposure of rats to secondhand smoke for 45 days causes decrease in perimysial collagen fibers, increase in the number of mast cells and increase in the immunolabeling for HIF-1α in lingual muscle cells. The present study was the first to evaluate the percentage of collagen, mast cell density and immunostaining for HIF-1α in rat tongues exposed to tobacco smoke.

Overexpression of hif-1α and morphological alterations in the tongue of rats exposed to secondhand smoke

Abstract Smoking is a risk factor for serious health problems and is associated with several changes in the tissues of the oral cavity. The aim of this study was to evaluate the collagen percentage, mast cells density, intensity of immunolabeled cells by anti-HIF-1α in the musculature lingual of rats exposed to secondhand smoke. Twenty-seven female Wistar albino rats were divided into three groups: rats not exposed to tobacco smoke inhalation (Control group) (n=7); rats exposed to smoke inhalation for 30 days (TAB 30) (n=10); and rats exposed to smoke inhalation for 45 days (TAB 45) (n=10). Subsequently, the animals were submitted to euthanasia and removal of the tongue for histological and immunohistochemistry processing and analysis. In the groups TAB 30 and TAB 45 there were a lower percentage of collagen, a higher density of mast cells and a greater intensity of anti-HIF-1α immunolabeled cells compared to Control group. There was also a positive and significant correlation between the percentage of collagen and mast cell density. There was not significative difference between TAB 30 e TAB 45 in any of the parameters evaluated. Therefore, the exposure of rats to secondhand smoke for 45 days causes decrease in perimysial collagen fibers, increase in the number of mast cells and increase in the immunolabeling for HIF-1α in lingual muscle cells. The present study was the first to evaluate the percentage of collagen, mast cell density and immunostaining for HIF-1α in rat tongues exposed to tobacco smoke.

Resumo O tabagismo é um fator de risco para sérios problemas de saúde e está associado a diversas alterações nos tecidos da cavidade oral. O objetivo deste estudo foi avaliar a porcentagem de colágeno, densidade de mastócitos e intensidade de células imunomarcadas por anti-HIF-1α na musculatura lingual de ratos expostos passivamente à fumaça principal do cigarro. Vinte e sete ratos Wistar albinos fêmeas foram divididos em três grupos: ratos não expostos à inalação da fumaça do tabaco (grupo controle) (n=7); ratos expostos à inalação da fumaça por 30 dias (TAB 30) (n=10); e ratos expostos à inalação da fumaça por 45 dias (TAB 45) (n=10). Posteriormente, os animais foram submetidos à eutanásia e remoção da língua para processamento e análise histológica e imuno-histoquímica. Nos grupos TAB 30 e TAB 45, houve diminuição do percentual de colágeno, maior densidade de mastócitos e maior intensidade de células imunomarcadas por anti-HIF-1α em comparação ao grupo controle. Houve também correlação positiva e significativa entre a porcentagem de colágeno e a densidade de mastócitos. Não houve diferença significativa entre TAB 30 e TAB 45 em nenhum dos parâmetros avaliados. Portanto, a exposição passiva de ratos à fumaça principal do cigarro por 45 dias provoca diminuição das fibras de colágeno perimisial, aumento do número de mastócitos e aumento da imunomarcação para o HIF-1α em células musculares linguais. O presente estudo foi o primeiro a avaliar a porcentagem de colágeno, densidade de mastócitos e imunomarcação para o HIF-1α em línguas de ratos expostos à fumaça do tabaco.

Periodontopathogenic microbiota, infectious mechanisms and preterm birth: analysis with structural equations (cohort-BRISA).

PURPOSE: The association between periodontopathogenic microbiota and preterm birth (PTB) has been overly studied. However, the biological mechanisms involved are little known. The objective is to evaluate the effect of periodontopathogenic bacteria burden (PBB), periodontal disease and other infections during pregnancy on preterm birth (PTB), through Structural Equation Modeling. METHODS: This was a case-control study nested in a prospective cohort called BRISA, including 330 pregnant women, 110 cases and 220 controls. This study included the following variables: cytokines interleukin-10 (IL-10) and transforming growth factor beta (TGF-ß), periodontal disease, PBB, age, socioeconomic status (SES), systemic infections and PTB. The correlations between variables were analyzed using Standardized Coefficient (SC). RESULTS: Greater PBB interfered positively with the occurrence of periodontal disease (SC: 0.027; p: 0.011), but these were not associated with the cytokines studied, nor with PTB. The lower serum levels of IL-10 (SC - 0.330; p 0.022) and TGF-ß (SC - 0.612; p < 0.001), and the presence of other systemic infections during pregnancy (SC 0.159; 0.049) explained the higher occurrence of PTB. CONCLUSION: It is possible that only the more severe periodontal disease and other systemic infections are capable of altering the cascade of cytokines regulating the inflammatory process and have an effect on the occurrence of PTB.

Use of Resin Infiltrate for the Treatment of White spot Lesions on Dental Enamel: Case Report / Utilização de Infiltrante Resinoso Para o Tratamento de Lesões de Mancha Branca do Esmalte Dental: Relato de Caso

Early diagnosis of white spot lesions allows non-invasive treatment to be indicated for the remineralization of active lesions. The goal was to report the clinical Protocol for the treatment of white spot lesion of a patient by the technique of resin infiltration, observing whether there is change of color on the white spot of the dental enamel after its treatment. The selected patient was 16 years old with good overall health, who featured white spot lesion of the dental enamel on tooth 13, classified as ICDAS score 2. The tooth was photographed, and its color was evaluated with the spectrophotometer in accordance with the CIELhC system. For comparison purposes, the color of tooth enamel in an area adjacent to the lesion was evaluated. The treatment of the lesion was performed with the resin infiltrate Icon (DMG, Hamburg, Germany), following the manufacturer's instructions. The color of the tooth was re-evaluated after the application of the resin infiltrate. At the end of the procedure, it was observed that the treatment of white spot lesion by resin infiltration technique reached its goal because, in addition to being minimally invasive, it was highly approved by patient and it provided the masking of the white spot lesion on the dental enamel. (AU).

O diagnóstico precoce de lesões de mancha branca permite que um tratamento não invasivo seja indicado para a remineralização das lesões ativas. O objetivo foi relatar o protocolo clínico para tratamento de lesão de mancha branca ativa de um paciente pela técnica do infiltrante resinoso, observando se existe alteração de cor da mancha branca do esmalte dental após o seu tratamento. Foi selecionada uma paciente, de 16 anos de idade, com boa saúde geral e que apresentava lesão de mancha branca no esmalte dental do dente 13, classificada pelo escore 2 do ICDAS. O dente foi fotografado e sua cor foi avaliada com o espectrofotômetro de acordo com o sistema CIELhC. Para fins de comparação, foi avaliada a cor do esmalte dental em uma área adjacente à lesão. O tratamento da lesão foi realizado com a resina infiltrante Icon (DMG, Hamburgo, Alemanha), seguindo as recomendações do fabricante. A cor do dente foi reavaliada após a aplicação do infiltrante. Ao final do procedimento, observou-se que o tratamento da lesão de mancha branca pela técnica da resina infiltrante atingiu seu objetivo pois, além de ter sido minimamente invasiva, foi bem avaliada pela paciente e proporcionou o mascaramento da lesão de mancha branca do esmalte dental. (AU).

Streptococcus mutans detection in saliva and colostrum samples.

OBJECTIVE: To detect Streptococcus mutans in colostrum and saliva of neonates and compare with its detection in saliva of mothers. METHODS: Forty-three healthy women, full-term gestations with no complications, submitted to elective Cesarean section, and their newborns were included in the study. Samples were investigated by polymerase chain reaction to detect S. mutans in genetic material from the samples. RESULTS: Approximately 16% of colostrum samples showed S. mutans , but not correlated with the presence of the bacteria in both samples of saliva. S. mutans was detected in 49 and 30% of saliva samples of mothers and neonates, respectively. There was a positive correlation in S. mutans detection between types of saliva. The number of maternal samples of saliva with detectable S. mutans was smaller in women receiving dental treatment during pregnancy. Tooth brushing, three times a day, influenced the detection of S. mutans in both the saliva and the colostrum. CONCLUSION: Although maternal saliva may present S. mutans , few samples of colostrum present the bacteria. The presence of bacteria in neonate saliva may be related to contact before birth. Dental treatment and hygiene habits seem to influence the detection of S. mutans in samples of maternal saliva and colostrum.

Tongue function, salivary flow rate and IgA, IgM and IgG total salivary levels in chronic chagasic patients

Although microscopic alterations have been detected in tongues and salivary glands of chagasic patients and the identification of biomarkers in saliva has proved advantageous, there are no studies evaluating tongue function and total salivary IgA, IgG and IgM levels in chronic chagasic patients. The aim of this study was to evaluate tongue function, salivary flow rate, and total salivary IgA, IgG and IgM levels comparing chronic and non-infected individuals. 37 patients were selected: chronic cardiac chagasic patients (n=6), chronic chagasic patients with the associated form of the disease (cardiopathy and megaesophagus) (n=11), and non-chagasic individuals (n=20). The tongue function underwent a phonoaudiological evaluation. The salivary flow rate was measured by sialometry. The total salivary IgA, IgG and IgM levels were evaluated by sandwich ELISA assay. Chagasic patients with the associated form of the disease presented higher salivary flow rate and lower salivary protein levels. No significant differences were noted in the lingual function or in the total salivary immunoglobulin levels among the groups. Although patients with chagasic megaesophagus presented higher levels of salivary flow and lower salivary protein, the fact that there were no significant differences in lingual function and total salivary immunoglobulin levels among the groups led to the conclusion that chronic chagas disease does not modify the lingual function or the total IgA, IgG and IgM salivary levels. The present study was the first to evaluate the function of the tongue and salivary total immunoglobulin levels in Chagas disease.

Streptococcus mutans detection in saliva and colostrum samples / Detecção de Streptococcus mutans em amostras de saliva e colostro

ABSTRACT Objective To detect Streptococcus mutans in colostrum and saliva of neonates and compare with its detection in saliva of mothers. Methods Forty-three healthy women, full-term gestations with no complications, submitted to elective Cesarean section, and their newborns were included in the study. Samples were investigated by polymerase chain reaction to detect S. mutans in genetic material from the samples. Results Approximately 16% of colostrum samples showed S. mutans , but not correlated with the presence of the bacteria in both samples of saliva. S. mutans was detected in 49 and 30% of saliva samples of mothers and neonates, respectively. There was a positive correlation in S. mutans detection between types of saliva. The number of maternal samples of saliva with detectable S. mutans was smaller in women receiving dental treatment during pregnancy. Tooth brushing, three times a day, influenced the detection of S. mutans in both the saliva and the colostrum. Conclusion Although maternal saliva may present S. mutans , few samples of colostrum present the bacteria. The presence of bacteria in neonate saliva may be related to contact before birth. Dental treatment and hygiene habits seem to influence the detection of S. mutans in samples of maternal saliva and colostrum.

RESUMO Objetivo Detectar Streptococcus mutans no colostro e na saliva de recém-nascido, e comparar com a detecção na saliva da mãe. Métodos Foram incluídos no estudo 43 mulheres saudáveis, com gestações a termo e sem complicações, que tiveram cesariana eletiva, e seus recém-nascidos. As amostras foram investigadas por reação de polimerase em cadeia para a detecção de S. mutans em material genético extraído das amostras. Resultados Cerca de 16% das amostras de colostro apresentaram S. mutans , não sem correlação com a presença das bactérias em ambas amostras de saliva. S. mutans foi detectado em 49 e 30% das amostras de saliva das mães e recém-nascidos, respectivamente. Houve correlação positiva na detecção de S. mutans entre os tipos de saliva. O número de amostras de saliva materna com S. mutans detectável foi menor nas mulheres que receberam tratamento odontológico durante a gravidez. A escovação três vezes ao dia influenciou na detecção do S. mutans tanto no saliva quanto no colostro. Conclusão Embora a saliva materna possa apresentar S. mutans , poucas amostras de colostro apresentam a bactéria. A presença de bactéria na saliva de neonatos pode estar relacionada ao contato antes do nascimento. O tratamento odontológico e os hábitos de higiene parecem influenciar na detecção de S. mutans em amostras de saliva e colostro maternos.

Efeito da Degradação Química sobre a Rugosidade Superficial e Estabilidade de Cor de Resinas Compostas Micro-híbrida e Nanoparticulada / Effect of Chemical Degradation on Surface Roughness and on Color Stability of Micro hybrid and Nanofilled Composites

A resina composta é o material restaurador mais utilizado atualmente. Apesar de seu sucesso clínico, o material está sujeito a alterações em suas propriedades físicas, quando exposto ao meio bucal. O objetivo foi avaliar a influência da degradação química na estabilidade de cor e na rugosidade superficial de resinas micro-híbrida e nanoparticulada. Foram confeccionados 40 discos (6,0mm x 2,0mm) da resina composta micro-híbrida (Z250XT) e 40 da resina nanoparticulada (Z350XT). Após a análise da cor inicial e da rugosidade inicial, 10 discos de cada resina foram imersos em saliva artificial (Controle, pH=6,4), suco de laranja (pH= 3,4), refrigerante de limão (pH=2,9) e vinho tinto (pH=3,1), por 4 horas por dia (37ºC) durante 30 dias. Após, foram realizadas as leituras finais de cor e rugosidade. Os dados obtidos para a estabilidade de cor foram analisados pelo Teste de Kruskal-Wallis, seguido pelo Teste de Dunn (α=5%). Para a análise da rugosidade foi utilizado o Teste t para amostras pareadas (α=5%). O manchamento provocado pela saliva artificial, suco de laranja e refrigerante foi similar, mas foi estatisticamente diferente do manchamento produzido pelo vinho, em ambas as resinas testadas. O vinho produziu o mesmo grau de manchamento nas resinas micro-híbrida e nanoparticulada. Foi observado que o suco de laranja, o refrigerante e o vinho promoveram aumento da rugosidade superficial dos discos de resina. Diante disso, concluiu-se que o vinho alterou a cor de resinas compostas micro-híbrida e nanoparticulada, e que a rugosidade superficial dos discos de resina foi alterada por todas as bebidas utilizadas. (AU).

Composite resin is the most used restorative material. Despite its clinical success, it is susceptible to changes in its physical properties when exposed to the oral environment. The aim was to evaluate the influence of acidic beverages on the color stability and on the surface roughness of the nanofilled and micro hybrid composite. Forty discs (6.0mm x 2.0mm) of the micro hybrid composite (Z250XT) and 40 of the nanofilled composite (Z350XT) were manufactured. After the measurement of the initial color and the initial roughness, 10 discs of each composite were individually immersed in artificial saliva (control, pH = 6.4), orange juice (pH=3.4), lime soda (pH = 2.9) and red wine (pH = 3.1) during 4 hours per day at 37°C for 30 days. At the end of the experiment, the final analysis of color and surface roughness were performed. The data obtained for the color stability were analyzed by Kruskal-Wallis and Dunn's test (α= 5%). For the analysis of the surface roughness, the t test for related samples was used (α= 5%). The color changes promoted by artificial saliva, orange juice and soda were similar, but they were statistically different from staining produced by wine, in both tested composites. Wine produced the same degree of staining in both composites. Orange juice, soda and red wine increased the surface roughness of the composite discs. It was concluded that red wine significantly changes the color of micro hybrid and nanofilled composites. Furthermore, all beverages increased the surface roughness of the composites tested in the study herein. (AU).

Tratamento de lesão de mancha branca com infiltrante resinoso: relato de caso / Resin infiltration of enamel white spot lesion: a case report

O diagnóstico precoce de lesões de mancha branca no esmalte dental permite que um tratamento não invasivo seja indicado para a remineralização das lesões ativas. O objetivo deste trabalho foi relatar um caso clínico onde foi utilizado um infiltrante resinoso para o tratamento não invasivo de uma lesão de mancha branca do esmalte dental. Foi selecionado um paciente com 14 anos de idade, do sexo masculino, leucoderma, com boa saúde geral, de alto risco à cárie, que apresentava lesão de mancha branca no incisivo central superior direito. Devido ao aspecto rugoso e opaco da lesão, optou-se pela realização do tratamento do dente com uma resina infiltrante (Icon®, DMG, Hamburg, Alemanha). Inicialmente foram realizadas fotografias intraorais e radiografia do dente acometido para fins de documentação do caso e futuras comparações. Em seguida, o dente recebeu profilaxia e posterior tratamento pela técnica da resina infiltrante, de acordo com as instruções do fabricante. Para a verificação da efetividade do procedimento, o paciente foi avaliado imediatamente e após 2 meses da conclusão do tratamento. Concluiu-se que a técnica da resina infiltrante de baixa viscosidade para o tratamento da lesão de mancha branca do dente 11 foi satisfatória, pois foi capaz de promover a paralização da doença e o mascaramento da lesão de mancha branca naquele dente.

The early diagnosis of enamel white spot lesions allows adopting a non-invasive treatment to remineralize active lesions. The objective of this study was to report a case where an infiltrant composite was used to treat enamel white spot lesion. A Caucasian 14- year-old male patient, with overall good health, and high-risk for dental caries was selected. He presented white spot lesion in the upper right central incisor. Due to the rough and opaque appearance of the lesion, the treatment with an infiltrant resin (Icon®, DMG, Hamburg, Germany) was chosen. Initially, intraoral photos and x-ray of the affected tooth have been were taken for documentation purposes and future comparisons. Then, the tooth was cleaned and treated with the infiltrant resin following the manufacturer's instructions. To verify the effectiveness of the procedure, the patient was assessed immediately and 2 months after treatment. In conclusion, the technique of low-viscosity infiltrant resin was satisfactory because it was able to impair the progression of the disease and to camouflage the white spot lesion on that tooth.

Levels of Pro and Anti-inflammatory Citokynes and C-Reactive Protein in Patients with Chronic Periodontitis Submitted to Nonsurgical Periodontal Treatment

Aim: to compare the levels of IFN-γ, TGF-ß and C-reactive protein (CRP) in healthy patients (HP) and chronic periodontitis patients (CP) before and seven days after the last session of Non-Surgical Periodontal Treatment (NSPT). Materials and Methods: 40 subjects were divided into two groups: healthy (n= 20), and with chronic periodontitis (n = 20). Serum and gingival crevicular fluid (GCF) were collected from each patient and quantified for IFN-γ, TGF-ß and CRP using the enzyme-linked immunosorbent assay (ELISA). Results: IFN-γ was found to be higher in the GCF of the CP group before NSPT in relation to the HP group (p<0.05), and it had significant higher levels after seven days of NSPT (p<0.05). The levels of TGF-ß in the GCF of CP patients before NSPT were significantly higher when compared to HP (p<0.05), but they decreased after seven days of NSPT (p>0.05). Serum CRP levels did not show statistical difference between CP and HP before or after NSPT. Conclusion: Therefore, our results demonstrated for the first time that NSPT causes early exacerbation of the immune response at the local level represented by increased levels of IFN-γ and decreased levels of TGF-ß in the gingival crevicular fluid after seven days of treatment.

Streptococcus mutans in Umbilical Cord Blood, Peripheral Blood, and Saliva from Healthy Mothers.

The aims of this study were to analyze the presence of Streptococcus mutans (SM)-DNA in cord blood (CB), maternal peripheral blood (PB), and maternal saliva (SA) and compare with data collected in health surveys. Sixty-four healthy women with pregnancies to term and without complications attending for elective cesarean section in the Clinical Hospital of Ribeirao Preto, Sao Paulo were included. Samples of PB and unstimulated SA were obtained on the day of hospitalization and samples of CB were collected after the delivery section. Samples were investigated using polymerase chain reaction for the presence of SM-DNA using specific primers. The results show over 50% of the sample of PB and CB showed SM-DNA detectable. There was a positive correlation between the SM detection in PB/CB and SA (P < 0.05). Pregnant women, who reported tooth brushing more than three times a day, often showed detectable SM-DNA in PB and CB (P < 0.05). In conclusion, the majority of children can have contact with SM-DNA during the intrauterine life by the CB. SM probably transferred from salivary habitat to PB and CB. The tooth brushing can be associated to S. mutans detection in blood samples.

Analysis of colostrum IgA against bacteria involved in neonatal infections.

OBJECTIVE: To describe e compare the specificity of IgA antibodies against bacteria extract of Klebsiella pneumoniae , Staphylococcus aureus , Escherichia coli , and Salmonella enteritidis . METHODS: Colostrum samples were aseptically collected in the first 12 hours after C-section delivery. The specificity of IgA against bacteria extracts was analyzed by the Western blot. RESULTS: The findings showed proteins of high molecular weight frequently detectable in the samples. S. aureus was the most frequently found bacterium in the samples (p<0.05). Approximately 93.8, 56.3, 62.5 and 60.4% of samples presented IgA reactive to S. aureus , K. pneumoniae , S. enteritidis, and E. coli, respectively. Roughly 40% of samples showed no IgA reactive to K. pneumoniae, S. enteritidis and E. coli . CONCLUSION: Clinical evidence of the importance of breastfeeding for the immune protection of neonates was consistent with the observed immunological findings, since most samples showed IgA reactive against the species tested. The application and development of immunotherapies during pregnancy, focused on frequently detected antigens, could be an important tool to enhance the presence of IgA in colostrum.

Analysis of colostrum IgA against bacteria involved in neonatal infections / Análise de IgA do colostro contra bactérias envolvidas em infecções neonatais

ABSTRACT Objective To describe e compare the specificity of IgA antibodies against bacteria extract of Klebsiella pneumoniae , Staphylococcus aureus , Escherichia coli , and Salmonella enteritidis . Methods Colostrum samples were aseptically collected in the first 12 hours after C-section delivery. The specificity of IgA against bacteria extracts was analyzed by the Western blot. Results The findings showed proteins of high molecular weight frequently detectable in the samples. S. aureus was the most frequently found bacterium in the samples (p<0.05). Approximately 93.8, 56.3, 62.5 and 60.4% of samples presented IgA reactive to S. aureus , K. pneumoniae , S. enteritidis, and E. coli, respectively. Roughly 40% of samples showed no IgA reactive to K. pneumoniae, S. enteritidis and E. coli . Conclusion Clinical evidence of the importance of breastfeeding for the immune protection of neonates was consistent with the observed immunological findings, since most samples showed IgA reactive against the species tested. The application and development of immunotherapies during pregnancy, focused on frequently detected antigens, could be an important tool to enhance the presence of IgA in colostrum.

RESUMO Objetivo Descrever e comparar a especificidade de anticorpos IgA de amostras de colostro contra extratos bacterianos de Klebsiella pneumoniae , Staphylococcus aureus , Escherichia coli e Salmonella enteritidis . Métodos As amostras de colostro foram coletadas assepticamente nas primeiras 12 horas após o nascimento por cesariana. A especificidade de IgA contra extratos de bactérias foi analisada por Western blot. Resultados Os achados mostraram proteínas de alto peso molecular frequentemente detectáveis nas amostras. S. aureus foi a bactéria mais encontrada nas amostras (p<0,05). Cerca de 93,8, 56,3, 62,5 e 60,4% das amostras apresentaram IgA reativa a S. aureus , K. pneumoniae , S. enteritidis e E. coli , respectivamente. Aproximadamente 40% das amostras não apresentaram IgA reativa contra K. pneumoniae , S. enteritidis e E. coli. Conclusão A evidência clínica da importância da amamentação para proteção imunológica ao recém-nascido foi consistente com os achados imunológicos observados, uma vez que a maioria das amostras mostrou IgA reativa contra as espécies testadas. A aplicação e o desenvolvimento de imunoterapias durante a gestação, focada nos antígenos frequentemente detectados, poderiam ser importantes instrumentos para aumentar a presença de IgA no colostro.

Comparisons of IgA response in saliva and colostrum against oral streptococci species.

The present study compared IgA specificity against oral streptococci in colostrum and saliva samples. Sixty-two mother-and-child pairs were included; samples of colostrum (C) and saliva (MS) were collected from the mothers and saliva samples were collected from babies (BS). The specificity of IgA against Streptococcus mutans and S. mitis were analyzed by western blot. Only 30% of babies' samples presented IgA reactivity to S. mutans, while 74 and 80% of MS and C, respectively, presented this response. IgA reactivity to S. mutans virulence antigens (Ag I/II, Gtf and GbpB) in positive samples showed differences between samples for Gtf and especially for GbpB (p < 0.05), but responses to Ag I/II were similar (p > 0.05). The positive response of Gtf-reactive IgA was different between C (90%) and MS (58%) samples (p < 0.05), but did not differ from BS (p > 0.05). GbpB was the least detected, with 48 and 26% of C and MS, and only 5% of BS samples presenting reactivity (p > 0.05). Eight percent of MS and C samples presented identical bands to SM in the same time-point. In conclusion, the differences of IgA response found between C and MS can be due to the different ways of stimulation, proliferation and transportation of IgA in those secretions. The colostrum has high levels of IgA against S. mutans virulence antigens, which could affect the installation and accumulation process of S. mutans, mainly by supplying anti-GbpB IgA to the neonate.

Comparisons of IgA response in saliva and colostrum against oral streptococci species

Abstract The present study compared IgA specificity against oral streptococci in colostrum and saliva samples. Sixty-two mother-and-child pairs were included; samples of colostrum (C) and saliva (MS) were collected from the mothers and saliva samples were collected from babies (BS). The specificity of IgA against Streptococcus mutans and S. mitis were analyzed by western blot. Only 30% of babies’ samples presented IgA reactivity to S. mutans, while 74 and 80% of MS and C, respectively, presented this response. IgA reactivity to S. mutans virulence antigens (Ag I/II, Gtf and GbpB) in positive samples showed differences between samples for Gtf and especially for GbpB (p < 0.05), but responses to Ag I/II were similar (p > 0.05). The positive response of Gtf-reactive IgA was different between C (90%) and MS (58%) samples (p < 0.05), but did not differ from BS (p > 0.05). GbpB was the least detected, with 48 and 26% of C and MS, and only 5% of BS samples presenting reactivity (p > 0.05). Eight percent of MS and C samples presented identical bands to SM in the same time-point. In conclusion, the differences of IgA response found between C and MS can be due to the different ways of stimulation, proliferation and transportation of IgA in those secretions. The colostrum has high levels of IgA against S. mutans virulence antigens, which could affect the installation and accumulation process of S. mutans, mainly by supplying anti-GbpB IgA to the neonate.

Effects of different desensitizing treatments on root dentin permeability.

The objective of this study was to evaluate the effects of diode laser and a desensitizing dentifrice on dentin permeability. Fifty-two root dentin fragments were obtained (5 × 5mm) and treated with 24% EDTA gel. The samples were divided into 4 groups (n = 13): G1, control (no treatment); G2, diode laser (λ = 908 nm, 1.5 W, continuous mode, 20s); G3, application of abrasive dentifrice for 1 minute (Elmex Sensitive Professional (International Gaba); and G4, application of abrasive dentifrice for 1 minute followed by irradiation with diode laser. Ten samples per group were immersed in 2% methylene blue solution for 4h. The specimens were washed, longitudinally sectioned, observed under optical microscopy, photographed and assessed based on the degree of dye leakage. The remaining samples were observed under scanning electron microscopy (SEM). The leakage data were subjected to ANOVA test, followed by Tukey's t-test (α = 5%). Groups 2, 3 and 4 showed less dye penetration than the control group (p < 0.05), but were similar among each other. SEM images showed that dentinal tubules were open in G1, and fused and occluded in G2. Group 3 showed dentinal tubules that were occluded by the metal ions from the toothpaste. G4 presented similar characteristics to G3, and the presence of fused dentin. The diode laser and the dentifrice were effective in reducing dentinal permeability, and the combination of the two treatments did not show better results than either one used alone.

A Utilização da Laserterapia para o Tratamento da Hipersensibilidade Dentinária: Revisão da Literatura / Laser Therapy in the Treatment of Dentin Hypersensitivity: Literature Review

A hipersensibilidade dentinária - HD é caracterizada por uma dor aguda, de curta duração, causada pela movimentação de fluidos no interior de túbulos dentinários expostos ao meio bucal. Recentemente, a utilização dos lasers de alta e baixa intensidade foi proposta como uma alternativa para o tratamento da HD. Este trabalho realizou uma revisão da literatura baseada em artigos publicados entre 2009 e 2016 sobre os efeitos dos lasers de baixa e alta intensidade para o tratamento da HD. A base de dados utilizada foi o PubMed utilizando-se os descritores: Dentin hypersensitivity, laser, Nd:YAG, Er:YAG, GaAlAs, Er,Cr:YSGG, CO2 , HeNe, e hydrodinamic theory. Foi observado que o tratamento da HD com lasers é um método biocompatível, não-invasivo e eficiente. Os lasers de baixa intensidade (HeNe e GaAlAs) estimulam as células nervosas do tecido pulpar, interferindo na polaridade das membranas celulares, pelo aumento da amplitude do potencial de ação. Esta ação leva ao bloqueio da transmissão do estímulo ao sistema nervoso central e estimula os odontoblastos a produzirem dentina reparadora, o que diminui a permeabilidade da dentina e interrompe a movimentação de fluidos nos túbulos dentinários. Os lasers em alta intensidade (Nd:YAG, Er:YAG, Er,Cr:YSGG e CO2) têm o objetivo de obliterar a embocadura dos túbulos dentinários, através da irradiação direta da dentina exposta ao meio bucal, promovendo a dissolução e a ressolidificação dos cristais de hidroxiapatita da dentina, levando à eliminação da sensibilidade dolorosa por um tempo prolongado. Pode-se concluir que os lasers de alta e baixa intensidade são efetivos para o tratamento da hipersensibilidade dentinária cervical.

Dentin hypersensitivity - DH is characterized by an acute and short-duration pain caused by the movement of fluids into the dentinal tubules that are exposed to the oral environment. Recently, the use of lasers has been proposed as an alternative for the treatment of DH. This study conducted a literature review based on papers published between 2009 and 2016 about the use of low and high intensity lasers for the treatment of HD. The database PubMed was consulted, using the following keywords: dentin hypersensitivity, laser, Nd : YAG, Er:YAG, GaAlAs, Er, Cr:YSGG, CO2 , HeNe, and hydrodinamic theory. It was observed that the treatment of DH with lasers can be a biocompatible, non-invasive, and efficient method. The low-intensity lasers (HeNe and GaAlAs) stimulate nerve cells in the pulp tissue, interfering with the polarity of the cell membranes by increasing the amplitude of the action potential, that inhibits the transmission of stimuli to the central nervous system. It stimulates the odontoblasts to produce reparative dentine, which decreases dentin permeability and impairs the movement of fluids into the dentinal tubules. The high-intensity lasers (Nd:YAG, Er:YAG, Er,Cr:YSGG and CO2) obliterate the dentinal tubules by direct irradiation of dentin that is exposed to the oral environment, promoting the dissolution and resolidification of hydroxyapatite crystals, leading to elimination of pain sensitivity for a long period. It can be concluded that the high and low intensity lasers are effective for the treatment of cervical dentine hypersensitivity